Coding

Part:BBa_K4228000:Design

Designed by: Keyao Zhao   Group: iGEM22_Tongji_China   (2022-09-27)


Bromelain from pineapple


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 312
    Illegal BamHI site found at 1
    Illegal XhoI site found at 646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 312
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutant amino acids, directed evolution, improve enzyme activity


Source

Sequence and Features

References